Composite

Part:BBa_K3258029:Design

Designed by: Ayush Pandit   Group: iGEM19_Stanford   (2019-10-21)


MDV + CAM Flipped with Terminator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 180
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 146
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 172
    Illegal XhoI site found at 168
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 180
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 180
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Designed with the addition of double terminator region to prevent leaky expression from the opposite antisense strand that results in functioning CAM resistance gene.


Source

Derived from CAM resistance gene in E. coli with MDV regions.

References